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oas2 e2g4k  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc oas2 e2g4k
    Oas2 E2g4k, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oas2+e2g4k/OAS2+Rabbit+mAb/pm41872511-231-36-57
    Average 94 stars, based on 12 article reviews
    oas2 e2g4k - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    RNA Sequencing:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Quantitative Proteomics:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Quantitative RT-PCR:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Derivative Assay:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Western Blot:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    In Vivo:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Injection:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Knock-Out:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Flow Cytometry:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Infection:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    HDAC Activity Assay:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Activity Assay:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Control:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.

    Binding Assay:

    Article Title: Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-I/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP), and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+ L) (catalog no. SA00001-2) and HRPconjugated Affinipure Goat Anti-Mouse IgG (H+ L) (catalog no. SA00001-1), were also from Proteintech.

    Article Title: Betrixaban Activates cGAS and ERVs to Promote Dual Nucleic-Sensing Antiviral Immunity
    Article Snippet: Rabbit antibodies against TBK1 (catalog no. 28397-1-AP), Beta Tubulin (catalog no. 10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech.10094-1-AP), RIG-1/DDX58 (catalog no. 25068-1-AP), MAVS (catalog no. 14341-1-AP) and IFIT3 (catalog no. 15201-1-AP), along with mouse antibodies targeting Beta Actin (catalog no. 66009-1-Ig) and the GFP tag (catalog no. 50430-2-AP), were procured from Proteintech. ... Additional antibodies, including rabbit phospho-TBK1/NAK (Ser172) (D52C2) (catalog no. 5483), phospho-IRF-3 (Ser396) (D6O1M) (catalog no. 29047), IRF-3 (D6I4C) (catalog no. 11904), cGAS (D1D3G) (catalog no. 15102), STING (D2P2F) (catalog no. 13647), MDA5 (D74E4) (catalog no. 5321), OAS2 (E2G4K) (catalog no. 24344), Viperin (F7T8D) (catalog no. 75654) and Acetyl-Histone H3 (Lys27) (D5E4) (catalog no. 8173) were obtained from Cell Signaling Technology.. Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.Secondary antibody utilized HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (catalog no. SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (catalog no. SA00001-1), were also from Proteintech.



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    The effect of TRPA1 antagonists on the mRNA expression of the IFN-stimulated antiviral genes MX1, <t>OAS2,</t> and DDX58 . A549 cells were cultured with IFN beta or IFN alfa-2a (both 10 ng/mL) in the presence or absence of the TRPA1 antagonists HC-030031 or A-967079 at indicated concentrations for 24 hours. Thereafter, total RNA was extracted, and RT-qPCR of MX1 , <t>OAS2,</t> and DDX58 was carried out against RPL30 as an endogenous control gene. Individual values and mean ± SD are presented, n = 5–6. Control was set as 1, and the other values are given in relation to that value. Statistical analysis was performed using one-way ANOVA with Holm- Šídák post-test, and comparisons were carried out against the IFN-treated condition (second bar from the left). ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote P < .05, <.01, <.001, or <.0001, respectively.
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    The effect of TRPA1 antagonists on the mRNA expression of the IFN-stimulated antiviral genes MX1, OAS2, and DDX58 . A549 cells were cultured with IFN beta or IFN alfa-2a (both 10 ng/mL) in the presence or absence of the TRPA1 antagonists HC-030031 or A-967079 at indicated concentrations for 24 hours. Thereafter, total RNA was extracted, and RT-qPCR of MX1 , OAS2, and DDX58 was carried out against RPL30 as an endogenous control gene. Individual values and mean ± SD are presented, n = 5–6. Control was set as 1, and the other values are given in relation to that value. Statistical analysis was performed using one-way ANOVA with Holm- Šídák post-test, and comparisons were carried out against the IFN-treated condition (second bar from the left). ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote P < .05, <.01, <.001, or <.0001, respectively.

    Journal: Molecular Pharmacology

    Article Title: Transient receptor potential ankyrin 1 promotes the expression of interferon-stimulated antiviral genes in human A549 lung epithelial cells

    doi: 10.1016/j.molpha.2025.100098

    Figure Lengend Snippet: The effect of TRPA1 antagonists on the mRNA expression of the IFN-stimulated antiviral genes MX1, OAS2, and DDX58 . A549 cells were cultured with IFN beta or IFN alfa-2a (both 10 ng/mL) in the presence or absence of the TRPA1 antagonists HC-030031 or A-967079 at indicated concentrations for 24 hours. Thereafter, total RNA was extracted, and RT-qPCR of MX1 , OAS2, and DDX58 was carried out against RPL30 as an endogenous control gene. Individual values and mean ± SD are presented, n = 5–6. Control was set as 1, and the other values are given in relation to that value. Statistical analysis was performed using one-way ANOVA with Holm- Šídák post-test, and comparisons were carried out against the IFN-treated condition (second bar from the left). ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote P < .05, <.01, <.001, or <.0001, respectively.

    Article Snippet: The following primary antibodies diluted 1:1000 in 5% BSA were used: OAS2 (E2G4K) Rabbit mAb #24344, MX1 (D3W7I) Rabbit mAb #37849, Rig-I (D33H10) Rabbit mAb #4200, Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649, and Stat1 antibody #9172 (all from Cell Signaling Technology, CST).

    Techniques: Expressing, Cell Culture, Quantitative RT-PCR, Control

    The effect of the TRPA1 antagonists on the protein expression of the IFN-stimulated antiviral genes MX1, OAS2 . and DDX58 . A549 cells were cultured with IFN beta (10 ng/mL; A, C) or IFN alfa-2a (10 ng/mL; B, D) for 24 hours with or without the TRPA1 antagonists HC-030031 (HC; 100 μ M) or A-967079 (A96; 100 μ M), total protein was extracted, and indicated proteins were detected by western blotting. In panels A and B, representative blots of the indicated proteins (with 2 biological replicates of each treatment) are shown following IFN beta (A) and IFN alfa-2a (B) treatment, respectively. Actin was detected as a loading control. Densitometric analysis is shown in panels C and D. Individual values and mean ± SD are presented, n = 5–6. IFN-treated conditions (second bar from the left) were set as 1, and the other values are given in relation to and statistically tested against that value. Statistical analysis was performed using one-way ANOVA with Holm- Šídák post-test. ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote P < .05, <.01, <.001, or <.0001, respectively.

    Journal: Molecular Pharmacology

    Article Title: Transient receptor potential ankyrin 1 promotes the expression of interferon-stimulated antiviral genes in human A549 lung epithelial cells

    doi: 10.1016/j.molpha.2025.100098

    Figure Lengend Snippet: The effect of the TRPA1 antagonists on the protein expression of the IFN-stimulated antiviral genes MX1, OAS2 . and DDX58 . A549 cells were cultured with IFN beta (10 ng/mL; A, C) or IFN alfa-2a (10 ng/mL; B, D) for 24 hours with or without the TRPA1 antagonists HC-030031 (HC; 100 μ M) or A-967079 (A96; 100 μ M), total protein was extracted, and indicated proteins were detected by western blotting. In panels A and B, representative blots of the indicated proteins (with 2 biological replicates of each treatment) are shown following IFN beta (A) and IFN alfa-2a (B) treatment, respectively. Actin was detected as a loading control. Densitometric analysis is shown in panels C and D. Individual values and mean ± SD are presented, n = 5–6. IFN-treated conditions (second bar from the left) were set as 1, and the other values are given in relation to and statistically tested against that value. Statistical analysis was performed using one-way ANOVA with Holm- Šídák post-test. ∗, ∗∗, ∗∗∗, and ∗∗∗∗ denote P < .05, <.01, <.001, or <.0001, respectively.

    Article Snippet: The following primary antibodies diluted 1:1000 in 5% BSA were used: OAS2 (E2G4K) Rabbit mAb #24344, MX1 (D3W7I) Rabbit mAb #37849, Rig-I (D33H10) Rabbit mAb #4200, Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649, and Stat1 antibody #9172 (all from Cell Signaling Technology, CST).

    Techniques: Expressing, Cell Culture, Western Blot, Control